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    The blood is anticoagulated during this procedure viagra overnight shipping canada with the use of sodium citrate. Immature hematopoietic progenitor cells including stem cells usually comprise less than 1% of the total cells. Harvesting of the stem cells is performed using apheresis, a procedure in which a machine continuously withdraws blood from one vein of the donor, removes the MNC fraction by centrifugation, and returns the remainder of the blood through another venous access. The cell product obtained in this manner contains predominantly immature granulocytes, lymphocytes, and monocytes.

    In patients who have stem cells mobilized with growth factors following chemotherapy, collections may be initiated when the number of CD34+ cells in the peripheral blood reaches the threshold established at each Chapter 4 / Transplantation of Stem Cells 59 institution. The adequacy of stem cell collection can be assessed by analyzing the percentage of cells expressing the CD34 antigen.

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